荧光定量PCR检测358例乙型肝炎病毒DNA结果分析

Analysis of detecting hepatitis B virus in 358 samples by fluorescence quantitative PCR

目的用荧光定量聚合酶链反应(FQ-PCR)定量检测血清中乙型肝炎病毒的数量以研究其临床价值。方法采用一种完全闭管式的PCR和荧光探针杂交技术相结合的实时检测定量PCR方法来检测358份临床血清标本。结果123份HBsAg,HBeAg,HBcAb阳性的标本,阳性率为96.7%(120例),平均拷贝数为1.8×108/ml;108份HBsAg,HBeAg,HBcAb阳性的标本其阳性率为39.8%(43例),平均拷贝数为6.6×105/ml;11份HBsAg,HBcAb阳性的标本,其阳性率为36.4%(4例),平均拷贝数为5.7×105/ml。结论FQ-PCR能够准确定量及有效避免假阳性。FQ-PCR可以真实反应HBV的感染和复制情况,对乙型肝炎的诊断,治疗方案的选择和疗效观察有较大的指导意义。

Objective To study the relationship of hepatitis B virus in serum, and its clinical significance by fluorescence quantitative polymerse chain reaction (FQ-PCR). Method FQ-PCR,which combines PCR and fluorescence probe hybridization,was used to measure HBV-DNA. Results In 123 samples with HBsAg+/HBeAg+/HBcAb+,FQ-PCR results were positive, with1.8×108/ml of HBV on average; In 108 samples with HBsAg+/HBeAg+/HBcAb+, the average was 6.6×105/ml with a positive rate of 39.8%; In 11 samples with HBsAg+/HBcAb+, the amount was 5.7×105/ml with a positive rate of 36.4%. Conclusions In-tube monitoring eliminates PCR cross-contamination which causes false positive, and real-time detection ensures accurate quantity. FQ-PCR can be used to monitor the true state of HBV infection and amplification.