小鼠异位小肠移植手术方式的改进

Modified surgical mode of paratopic intestine transplantation in mice

目的建立良好的小肠移植动物模型,用于进一步作小肠移植免疫及相关研究。方法采用显微外科技术,获取带门静脉和腹主动脉的供体小鼠小肠,移植物腹主动脉离体修剪成带肠系膜上动脉的动脉袖片(Carrel'spatch),将移植物门静脉和Carrel’spatch分别与受体下腔静脉与腹主动脉作端侧吻合。移植肠的远端与受体近端空肠作端侧吻合,近端结扎封闭。结果实验初期移植成功率为37.9%,后期技术成熟后移植成功率为81.9%,供体手术时间为(60±10)min,移植肠冷保存时间为(20±5)min,受体与移植物血管吻合时间为20～25min。结论采用改进手术方式制作的肠道内引流小鼠小肠移植模型稳定,是研究小鼠同种异体小肠移植免疫有用的实验工具。

Objective To develop a murine paratopic model of intestinal t ransplantation for study the immune response to allograft. Methods Under the op erational microscope, donor intestinal graft was harvested along with vascular p edicles, and the graft portal vein and superior mesenteric artery were anastomos ed with inferior vena cava and infrarenal abdominal aorta respectively. The dist al end of intestinal graft was connected to the proximal jejunum of recipient in an end-to-side way, whereas the proximal end of the graft was closed by ligatio n. Results The successful rate of the initial 58 mice SBTx was 37.9%, whereas t hat for the final 94 animals was 81.9%.The average duration of the donor procedu re was 60±10min and the average cold storage time for the graft was 20±5min, t he graft revascularization took 20 to 25 min. Conclusion The murine intestinal transplantation model with internal drainage of graft intestinal contents have a high survival rate for following experimental application.