HPLC法测定连翘提取物中连翘苷含量

The HPLC's law measurement Fructus Forsthiae collects in the matter Phillyrin content

目的:测定连翘提取物中连翘苷的含量。方法:用聚酰胺色谱柱对连翘提取物进行纯化,用 HPLC 法进行含量测定,色谱柱,ODS—C18,流动相为甲醇—水(40:60),检测波长280nm。结果:平均加样回收率为99.32%。结论:该方法简便、快速、灵敏性高,结果准确可靠,可用于连翘苷含量控制。

Objective measurement Fructus Forsthiae collects in the matter content of Phillyrin Methods:Collecing the matter to the Fructus Forsthiae and carrying on the purification in the way of the polyamide color spectrum post,and carries on content measurement in the way of HPLC's law,ODS—C_(18) going from place to placely one another act as methanol—water(40: 60),examination wavelength 280nm.Result:Equally plusing a type rate of recovery act as 99.32%.Conclusion:Thiat means is portably an speedily and the maneuverability is high,and the result is accurately dependable,and usablely dominates to Phillyrin content.