二氯乙酸对小鼠肝细胞DNA损伤效应研究

STUDIES ON DNA DAMAGE OF MICE HEPATOCYTES INDUCED BY DICHLOROACETIC ACID.

目的 :研究二氯乙酸的 DNA损伤作用。方法 :小鼠肝细胞的体外、体内试验。体外试验 ,取小鼠肝细胞染毒 ,染毒 1h后彗星试验 ;体内试验 ,小鼠连续灌胃 6 d,每天一次 ,在第 1、 3、 6 d时各染毒 3h后取其肝细胞进行彗星试验。荧光显微镜下观察、计数拖尾细胞的尾长。结果 :无论是体外试验 ,还是体内试验 ,二氯乙酸均可导致肝细胞 DNA损伤 ;体内试验结果显示 ,随染毒次数的增加 ,DNA损伤加重。结论 :二氯乙酸是肝细胞的 DNA损伤剂 ,致癌作用可能与其 DNA损伤作用有关 ,属于遗传致癌物。

Objective:To study on the DNA damage of dichloroacetic acid(DCA).Methods:Assay of mice hepatocytes in vitro and in vivo.In vitro,the mice hepatocytes were treated with DCA for 1 hour and then were tested by comet assay;In vivo,DCA were administerd to mices one time in a day lasting six days.On first day,third day and sixth day,mices were killed three hours after they had been treated.From each mice,a piece of liver was cut for comet assay.Tail length of the cells were observed and counted through fluorescence microscope.Results:Not only in vitro,but also in vivo,DCA can lead to DNA damage of mice hepatocytes.And in vivo,with times of DCA treating increased,DNA damage aggravated.Conclusion:DCA is an agent of DNA damage to hepatocyte,and belongs to genetic carcinogen.Its carcinogenesis may be related with its action of inducing DNA damage.