肺表面活性物质对哮喘大鼠血清一氧化氮、丙二醛含量及超氧化物歧化酶活性的影响

The effect of pulmonary surfactant on nitric oxide,superoxide dismutase and malonic dialdehyde in serum of rat asthma model

目的 观察肺表面活性物质 (PS)对大鼠哮喘模型中一氧化氮 (NO)、丙二醛 (MDA)含量及超氧化物歧化酶 (SOD)活性的变化 ,探讨PS治疗哮喘的机制。方法 设立正常组、哮喘组、生理盐水 (NS)处理组及PS处理组 ,建立大鼠哮喘模型 ,检测血清中NO-2 /NO-3 、MDA含量及SOD活性。结果 正常大鼠的血清NO-2 /NO-3 、MDA及SOD分别为 (10 6 73± 34 96 ) μmol/L、(17 79±5 6 8)nmol/mL、(81 80± 7 93)U/mL ;哮喘组分别为 (410 2 3± 86 77) μmol/L、(32 4 2± 6 11)nmol/mL、(34 72± 12 95 )U/mL ;NS处理组分别为 (45 2 86± 85 2 7) μmol/L、(34 0 8± 7 83)nmol/mL、(2 8 89±13 19)U/mL ;PS处理组分别为 (2 70 19± 82 0 9) μmol/L、(2 6 2 4± 5 92 )nmol/mL、(78 5 2± 7 11)U/mL。哮喘组血清中NO-2 /NO-3 和MDA含量高于正常组 (P <0 0 1) ,SOD活性低于正常组 (P <0 0 1) ;NS处理组与哮喘组比较各项指标均无显著性差异 (P >0 0 5 ) ;PS处理组血清SOD活力明显高于哮喘组 (P <0 0 1) ;NO-2 /NO-3 和MDA含量显著低于哮喘组 (P <0 0 1) ;PS处理组NO-2 /NO-3 和MDA含量显著高于正常组 (P <0 0 1) ,SOD活性与正常组比较无显著性差异 (P >0 0 5 )。结论 PS干预可影响哮喘大鼠体内氧化

Objective To investigate the effect of pulmonary surfactant (PS) on nitric oxide (NO)superoxide dismutase (SOD),and malonic dialdehyde (MDA) in rat asthma model.Methods The rats were randomly divided into four groups,ie.normal group,asthma group,normal saline group and PS group.Serum NO- 2/NO- 3,MDA levels and SOD activity were measured.Results Serum NO- 2/NO- 3,MDA levels and SOD activity in four groups were as follows:(106.73±34.96)μmol/L,(17.79±5.68)nmol/mL,and (81.80±7.93)U/mL in normal group;(410.23±86.77)μmol/L,(32.42±6.11)nmol/mL,and (34.72±12.95)U/mL in asthma group (452.86±85.27)μmol/L,(34.08±7.83)nmol/mL,and (28.89±13.19)U/mL in NS group;(270.19±82.09)μmol/L,(26.24±5.92)nmol/mL,and (78.52±7.11)U/mL in PS group.Serum NO- 2/NO- 3 and MDA levels in asthma group were significantly increased compared with those in normal group (P<0.01),while serum SOD activity was significantly decreased (P<0.01).There was no remarkable difference between NS group and asthma group.Serum SOD activity in PS group was higher than that in asthma group (P<0.01),on the contrary,serum NO- 2/NO- 3 and MDA levels were lower than those in asthma group (P<0.01).Serum NO- 2/NO- 3 and MDA levels in PS group were significantly increased compared with those in normal group (P<0.01),but there was no remarkable difference of serum SOD activity between these two groups (P>0.05).Conclusion The changes of NO,SOD and MDA in rat asthma model after administration of PS suggest that PS can improve the imbalance of oxidant/antioxidant system in rat asthma model,which indicates that PS might play an important role in the treatment of asthma in future.