β_3肾上腺能受体基因与肥胖、糖尿病、胰岛素抵抗关系的群体水平研究

Relationship between β_3-AR gene and obersity, type 2 diabetes, insulin resistance in Chinese hans population

目的 研究单纯性肥胖、2型糖尿病(T2DM)、胰岛素抵抗与β3-肾上腺能受体(β3-AR)基因之间的关系。方法 运用多聚酶链反应-限制性片段多态性分析(PCR-RFLP)技术分析了53例正常对照者、105例单纯性肥胖患者、63例T2DM非肥胖患音和114例T2DM伴肥胖患者的β3-AR甚因外显子64位色氨酸(Trp)、精氨酸(Arg)的多态性。结果 具有Arg等位基因者,其腰臀围比(WHR)、平均血压(MBP)、收缩压(SBP)、舒张压(DBP)、空腹血糖(FBS)、餐后血糖(PBS)、空腹胰岛素(FINS)、餐后胰岛素(PINS)、空腹C肽(FCP)、餐后C肽(PCP)均比Trp纯合组显著增加,而胰岛素敏感指数(ISI)明显降低;在非T2DM,伴有高胰岛素血症的肥胖亚组的Arg等位基因频率明显升高。结论Trp/Arg多态性的改变导致了胰岛素抵抗、肥胖、T2DM的发生。β3-AR基因是中国汉族人群胰岛素抵抗、肥胖、T2DM的候选基因。

Objective To explore the relationship between β3 -adrenergic receptor(β3-AR)gene and obesity,T2DM,insulin resistance in Chinese Hans population. Methods 53 health individuals, 105 simple obesity subjects. 63 T2DM patients without obesity, and 114 T2DM with obesity have been detected with the technique of PCR-RFLP in codon 64 of the exon region of β3-AR gene representing the variation Trp/Arg. Results Comparison with the subjects of Trp homozygous group, the individuals with Arg allele were much higher in WHR,MBP,SBP,DBF,FBS,PBS,FINS,PINS,FCP,PCP and lower in ISI. Frequency of Arg allele was higher in HINS subgroup without T2DM. Conclusion The results indicate that the Trp/Arg variation might lead to insulin resistance,obesity and T2DM β3-AR gene is the candidate gene of insulin resistance,obesity and T2DM in Chinese Hans population.