摘要
目的 观察HO CO系统在肝硬化病人肝组织中的表达及与门静脉压力的关系 ,以探讨其在肝硬化门脉高压中的作用。方法 随机选取 2 0例正常志愿者及 2 0例肝硬化患者 ,在B超引导下经皮经肝穿刺分别测定门静脉压力、抽取门静脉血和外周血并留取肝组织 ,测定血液中CO浓度 ,用免疫组化和RT PCR方法观察肝组织HO 1及其HO 1mRNA的表达。结果 肝硬化病人下腔静脉及门静脉血中CO浓度、肝组织HO 1、HO 1mRNA的表达及门静脉压力均分别显著高于正常对照组 ,正常对照组的外周血和门静脉血中CO浓度水平接近 ,无明显差异 ;但肝硬化患者的门静脉血CO浓度显著高于外周血CO浓度。结论 门脉血CO浓度、肝组织中HO 1以及HO
Purpose To observe the relationship between portal pressure and the expression of HO-CO system in liver of patients with hepatic cirrhosis and the effect on portal hypertension in hepatic cirrhosis. Methods Twenty normal volunteers and 20 patients with hepatic cirrhosis were randomly selected under the direction of B-ultrasonic equipment and percutaneous liver puncture was performed to test portal pressure, to drain blood from peripheral vein and portal vein and liver tissue was taken in order to test the density of CO in blood.The expression of HO-1、HO-1mRNA of liver was measured by RT-PCR. Results The density of CO in peripheral and portal vein blood, expression of HO-1 and HO-1mRNA in the patients was significantly higher than it was in control group. Comparing with control group, there was no obvious difference in CO density between peripheral and portal vein blood; but to patients, the latter was significantly higher than the former. Conclusions The portal pressure was closely related to CO density in portal vein, expression of HO-1 and HO-1mRNA in liver tissue of patients.
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2004年第5期481-483,共3页
Fudan University Journal of Medical Sciences
