榄香烯乳对结肠癌Lovo细胞端粒酶活性、细胞凋亡及细胞周期的影响

Effect of Elemene on the Telomerase Activity ,Apoptosis and Cell Cycles of the Colon Cancer Lovo Cell Line

目的 :探讨榄香烯乳对结肠癌Lovo细胞的作用机制。方法 :采用不用浓度的榄香烯乳处理结肠癌Lovo细胞后 ,分别应用四唑蓝比色试验、端粒重复扩增 -微孔板杂交法、琼脂糖凝胶电泳方法及流式细胞仪研究榄香烯乳对结肠癌细胞生长、端粒酶活性、凋亡及细胞周期的影响。结果 :榄香烯乳对结肠癌Lovo细胞增殖具有较强的抑制作用 ,能够抑制端粒酶活性 ,诱导细胞凋亡 ,且这种作用呈浓度及时间依赖 ;且细胞阻滞于G0 G1 期。结论 :榄香烯乳抑制结肠癌Lovo细胞恶性增殖与抑制端粒酶活性、诱导凋亡及细胞周期阻滞有关。

Objective:To probe into the effect of elemene on the telomerase activity,apoptosis and cell cycle of the colon carcinoma Lovo cell line. Methods:After the Lovo cells had been treated with elemene in different concentrations, the effects of elemene on the growth rate, telomerase activity,apoptosis and cell cycle of the cancer cells were studied with MTT assay,telomeric repeat amplification protocol(TRAP)-microtiter plate hybridization, agarose gel electrophoresis and flow cytometry, respectively. Results:Elemene was shown to have a relatively strong inhibitory effect on the proliferation of Lovo cells. Elemene could also suppress the telomerase activity and induce cell apoptosis in a concentration and time-dependent manner. Besides, the cancer cells were found to be blocked in the G 0/G 1 phase. Conclusion:The inhibition of the malignant multiplication of human colon cancer Lovo cells by elemene may be related to the suppression of the telomerase activity, induction of apoptosis and blockade of the cancer cells in the G 0/G 1 phase.