羟基磷灰石/磷酸三钙作为细胞载体的体外实验(英文)

Hydroxyapatite/tricalcium phosphate matrix scaffold as cell carriers in vitro

目的 :探讨羟基磷灰石 /磷酸三钙 (HA/TCP)复合材料作为组织工程载体的可能 ;为基质材料表面改性提供实验基础。方法 :应用人骨髓基质细胞 (hBMSCs)和脐静脉内皮细胞 (UVECs)和HA/TCP进行混合培养 ,应用光学显微镜、荧光显微镜及扫描电子显微镜对其进行观察。结果 :人骨髓基质细胞和脐静脉内皮细胞在HA/TCP表面均生长良好 ,并可观察到细胞长入基质材料微孔内的改变。结论 :羟基磷灰石 /磷酸三钙 (HA/TCP)复合材料可作为细胞移植的载体。

Objective To explore the possibility of hydroxyapatite/tricalcium phosphate(HA/TCP)matrix scaffold as cell carriers in tissue engineering,and to provide a direct evidence for the resurfacing of matrix scaffold. Methods Human bone marrow stromal cells (hBMSCs) and umbilical vein endothelial cells(UVECs) were co cultured on the surface of matrix scaffold.The morphologic characters during culture on HA/TCP matrix scaffold′s surfaces were checked by light microscope,fluorescence microscope,and scanning electronic microscope.Results Both human bone marrow stromal cells and human umbilical vein endothelial cells showed good biocompatibility with HA/TCP matrix scaffold.The cell growth into the micropore of HA/TCP matrix scaffold could be seen under both fluorescence microscopy and scaning electron microscopy.Conclusion HA/TCP matrix scaffold can be used as a vehicle for the cell transplantation.