67例面中线非霍奇金淋巴瘤临床病理及其EBV感染关系

The Clinicopathology of Non-Hodgkin's Lymphoma in the Midline and Its Relationship with Epstein-Barr Virus Infection

目的 探讨面中线非霍奇金淋巴瘤 (Non -Hodgkin’slymphma ,NHL)的临床病理特点及其与EBV感染的关系。 方法 应用免疫组化SP法检测多种抗体标记T淋巴细胞 (CD45RO、CD5 6、TIA -1)和B淋巴细胞 (CD2 0、CD45RA) ,同时进行EBV原位杂交检测。 结果 ⑴ 67例病例中 ,男女之比为 2 .7∶1,平均年龄 43岁 ,发病部位多位于鼻腔 ;⑵按照WHO新分类法 ,67例中B细胞淋巴瘤有 3 1例 ( 4 7.4% ) ,NK/T细胞淋巴瘤有 3 0例 ( 4 6.2 % ) ,外周T (非特殊类 )细胞淋巴瘤有 6例 ( 9.1% ) ;⑶ 67例中有 2 7例EBER1/ 2检测表达阳性 ( 4 0 .3 % ) ,其中 2 5例为NK/T细胞淋巴瘤 ,2例为外周T(非特殊类 )细胞淋巴瘤 ,3 1例B细胞淋巴瘤均表达阴性。 结论 面中线NHL以NK /T细胞淋巴瘤多发 ,EBV感染与淋巴瘤组织类型有关 ,与部位无关。

Objective To study the clinicopathology of non-Hodgkin's lymphoma of the midline and its relationship with EBV infection. Methods In situ hybridization for EBER1/2, immumohistochemical straining by three steps ABC technique for TIA-1,CD56,CD20,CD45RO and so on. Result (1) in 65 cases of non-Hodegkin's lymphomas of the midline,the ratio of male to female was 2.7 to 1.The average age was 43 years old. The nasal cavity was the predilection site of NHL. (2) According to the WHO classification,of 67 cases,31 cases (47.7%) were confirmed as B cell lymphoma,30 cases (46.2%) as NK/T cell lymphoma ,6 cases (9.1%) as T cell lymphoma. ⑶in 27 of the 67 cases(40.3%), EBER1/2 signals were detected in the tumor cells. EBER1/2 were detected in 25 cases of NK/T cell lymphomas and in 2 cases of T cell lymphomas. EBER1/2 were not detected in 31 cases of B cell lymphomas. Conclusion The lymphomas of the midline are more often occurred as NK/T cell lymphomas, the infection of EBV has a relationship with the histology type of lymphomas, not with the site.