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T细胞膜脂肪微区域与白细胞介素-2α受体 被引量：2

Interleukin-2α receptor in membrane lipid rafts
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摘要 目的 :确定T细胞膜脂肪微区域 (lipidrafts )是IL 2Rα的功能性区域。　方法 :应用流式细胞仪检测T细胞表面分子CD2 5 (IL 2Rα)的表达 ,分离T细胞膜脂肪微区域 ,应用蛋白质印迹法检测分析IL 2Rα所在的T细胞膜脂肪微区域分离组分。　结果 :在IL 2刺激下 ,T细胞膜表面分子CD2 5 (IL 2Rα)阳性表达率为 37.0 8% ,蛋白质印迹法检测确定IL 2Rα存在于脂肪微区域组分。　结论 :T细胞膜脂肪微区域是IL 2Rα的功能性区域。 Objective: To determine lipid rafts on the membrane of T cells is the functional microdomains for IL-2α receptor. Methods: Flow cytometric analysis was performed for expression of CD25(IL-2Rα) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Rα in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Results: cells were stimulated with IL-2 and expression of CD25(IL-2Rα) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Rα was localized in lipid rafts. Conclusions: lipid rafts is the functional microdomains for IL-2α receptors.
作者 李秋荣 马健 汪灏 黎介寿
机构地区 南京军区南京总医院解放军普通外科研究所
出处 《肠外与肠内营养》 CAS 2004年第5期262-264,共3页 Parenteral & Enteral Nutrition
基金 国家重点基础研究发展规划 ( 2 0 0 3CB5 15 5 0 2 ) 南京军区南京总医院科研基金 ( 2 0 0 40 3 4)项目
关键词 T细胞 IL-2RΑ 脂肪 Α受体 蛋白质印迹法 CD25 白细胞介素-2 Lipid rafts Interleukin-2α receptor Immunoblotting
分类号 Q26 [生物学—细胞生物学]
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参考文献9

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同被引文献53

  • 1黄敏珊,黄炜,吴其年,罗惠玲,张志凌,张东方,杨凤仪.齐墩果酸诱导人乳腺癌细胞凋亡及与细胞内Ca^(2+)水平关系的研究[J].中国现代医学杂志,2004,14(16):58-60. 被引量：22
  • 2唐雪莲,李静,耿美玉.整合素与肿瘤转移[J].中国药理学通报,2005,21(10):1164-1167. 被引量：12
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肠外与肠内营养
2004年 第5期

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