摘要
目的 :确定T细胞膜脂肪微区域 (lipidrafts )是IL 2Rα的功能性区域。 方法 :应用流式细胞仪检测T细胞表面分子CD2 5 (IL 2Rα)的表达 ,分离T细胞膜脂肪微区域 ,应用蛋白质印迹法检测分析IL 2Rα所在的T细胞膜脂肪微区域分离组分。 结果 :在IL 2刺激下 ,T细胞膜表面分子CD2 5 (IL 2Rα)阳性表达率为 37.0 8% ,蛋白质印迹法检测确定IL 2Rα存在于脂肪微区域组分。 结论 :T细胞膜脂肪微区域是IL 2Rα的功能性区域。
Objective: To determine lipid rafts on the membrane of T cells is the functional microdomains for IL-2α receptor. Methods: Flow cytometric analysis was performed for expression of CD25(IL-2Rα) on the surface of T cells. Lipid rafts were isolated by discontinuous sucrose density gradient ultracentrifugation. The localization of IL-2Rα in fractions isolated from sucrose density gradients was determined by immunoblotting and detected by chemiluminescence. Results: cells were stimulated with IL-2 and expression of CD25(IL-2Rα) on the surface of T cells was 37.08%. Immunoblot analysis of fractions from sucrose gradients revealed that a large proportion of IL-2Rα was localized in lipid rafts. Conclusions: lipid rafts is the functional microdomains for IL-2α receptors.
出处
《肠外与肠内营养》
CAS
2004年第5期262-264,共3页
Parenteral & Enteral Nutrition
基金
国家重点基础研究发展规划 ( 2 0 0 3CB5 15 5 0 2 )
南京军区南京总医院科研基金 ( 2 0 0 40 3 4)项目