摘要
本文以绿原酸、咖啡酸为含量测定指标，采用HPLC法测定了不同产地忍冬藤中绿原酸、咖啡酸含量。色谱柱为Diamonsil^TMC18,4.6mm×250mm，10μm柱，流动相为乙腈-0.2%磷酸溶液(11：89)，检测波长为324nm。线性范围：绿原酸为0．08～0．40μg，r=0．9995，咖啡酸为0．03～0.15μg，r=0．9996；加样回收率：绿原酸为100．79％，RSD=2.17％，咖啡酸为98.05％，RSD=2.44％。本法快速、简便，重现性好，可用于控制忍冬藤的内在质量。
An HPLC method for determination of the content of chlorogenic acid and caffeic acid in bines of Lonicera Japonica Thurtb. from different sources was established using DiamonsilTMC18(4. 6mm× 250mm, 10μm)column. The mobile phase was HCN-0. 2%H3PO4(11 : 89)and the detection wavelength was 324nm. The calibration curve of chlorogenic acid was linear in the range of 0. 08-0. 40μg,r = 0. 9995. The mean recovery of chlorogenic acid was 100. 79% ,RSD% = 1. 4%. The calibration curve of caffeic acid was linear in the range of 0. 03-0. 15μg,r=0. 9996. The mean recovery of caffeic acid was 98. 05%, RSD% = 2. 44%. This method was found to be convenient,quick with good reproducibility. It can be used as a method of quality control.
出处
《中国药品标准》
CAS
2004年第4期47-50,共4页
Drug Standards of China
