摘要
目的:建立参鹿扶正合剂中五味子醇甲的高效液相色谱测定方法。方法:用十八烷基硅烷键合硅胶为填充剂;甲醇-水(65:35)为流动相;检测波长为250nm;样品以水饱和的正丁醇提取。结果:五味子醇甲在0.614~3.070μg范围内呈良好的线性关系,r=0.9996,加样回收率为98.05%,RSD=2.22%(n=5)。结论:本法简单可行,结果准确,重现性良好。
Objective: To develop an assay for the determination of Schisandrin in Shenlufuzheng Oral Solution. Methods: A RP-HPLC was used on C18 column with a mobile phase of methanol-water(65 : 35) and the detection wavelength at 250nm. Result;The standard curves for Schisandrin were linear in the range of 0. 614-3. 070μg(r = 0. 9996) ;The mean recoveries of Schisandrin were 98. 05% with RSD=2. 22%(n = 5). Conclusions:The method is convenient and rapid. The result is accurate and reproducible.
出处
《中国药品标准》
CAS
2004年第4期61-62,共2页
Drug Standards of China