白腐菌Pleurotus ostreatus漆酶对蒽醌染料SN4R脱色研究

Study of decolorization of anthraquinone dye SN4R by laccase from white-rot fungi Pleurotus ostreatus

利用白腐菌Pleurotusostreatus324粗漆酶和纯漆酶进行蒽醌染料SN4R的脱色研究.粗漆酶可使蒽醌染料SN4R脱色,最适脱色pH、温度和漆酶酶活分别为4.0、40℃和30IU/mL,12h染料脱色率为55%.纯漆酶不能使SN4R脱色,但小分子介体物质可介导纯漆酶对染料SN4R的氧化脱色,在最适条件下4h染料脱色率可达80.6%.粗漆酶添加适当浓度的介体物质,可使染料完全脱色.因此,小分子还原介体物质的存在有助于染料废水的降解和脱色.

The decolorization of anthraquinone dye SN4R was studied with crude and pure laccase from white-rot fungi Pleurotus ostreatus strain 324. Anthraquinone dye SN4R can be decolorized by crude laccase, the optimal decolorization pH, temperature and laccase activity are 4.0, 40 ℃ and 30 IU/mL respectively, the decolorization rate is 55% after 12 hour treating. Anthraquinone dye SN4R can not be decolorized by pure laccase, but when small molecular mediator is added in enzyme system, the decolorization can be carried through, and the decolorization rate can achieve to 80.6% after 4 hours treating under the optimal condition. Anthraquinone dye SN4R can be completely decolorized using crude laccase and mediator. It is beneficial to dye wastewater decolorization and degradation by adding small molecular mediator into reaction system.