三磷酸肌醇抗油脂氧化性的研究

Antioxidant Effect of Inositol Triphosphate In Oil

本文通过在微波条件下 ,控制水解条件 ,当水解度达到 5 0 %时 ,将水解混和物通过装有 717型强阴离子树脂柱 ,并用 0 .0 5∽ 0 .7M的盐酸进行线性梯度洗脱 ,洗脱物分别取样消化 ,检测无机磷含量变化 ,并将同一峰值部分收集在一起 ,分离得到低磷酸肌醇各组分。各组分用HPLC和MS进行了确证。同时研究了三磷酸肌醇的抗油脂氧化活性。通过对菜籽色拉油在 6 0℃生化恒温箱中强化贮存 ,通过添加不同浓度的三磷酸肌醇 ,并用植酸 ,BHT作对照 ,用碘量法定期测定菜籽油的过氧化值 (POV值 ) ,用 2 ,4 -二硝基苯肼法测羰基值 ,并测定了酸值。实验发现三磷酸肌醇对菜籽油具有明显的抗氧化作用 ,但酸值有所升高。

Procedure for preparing inositol triphosphate from sodium phytate was established. Hydrolysis was achieved by microwave radiation. When the hydrolysis rate of phytic acid was about 50%, the hydrolyzed solutions were pumped into a column containing 717 strong anion-exchange resin, then a linear gradient of 0.05∽0.7M HCl was delivered. The samples of each tube were digested and the phosphate was determined. The contents of tubes corresponding to the same individual inositol phosphate were pooled and rotary evaporated under vacuum. Each inositol phosphate was identified by HPLC and MS. The antioxidant effect of inositol triphosphate was evaluated by oven test with rapeseed oil at 60℃. Phytic acid and BHT were tested in parallel for comparison. The oil oxidation was followed by measuring the quantity of primary oxidation products (POV) by way of iodometry, measuring carbonyl value by way of 2,4-dinitrophenylhydrazine, and also measuring oil acidity. The result indicates that the inositol triphosphate has high antioxidant effect in rapeseed oil, but results in the oil acidity a little higher.