恶性黑素瘤和普通痣细胞痣细胞外信号调节激酶途径相关蛋白的研究

The Study of ERK Pathway Related Protein Expression in Malignant Melanoma and Ordinary Nevi

目的探讨恶性黑素瘤(MM)和普通痣细胞痣细胞外信号调节激酶(ERK)途径相关蛋白ERK1和ERK2以及细胞周期素D1(CyclinD1)蛋白的表达情况,分析这3种蛋白与MM的临床病理关系。方法采用免疫组化SP法对30例MM和13例普通痣细胞痣中ERK1、ERK2和CyclinD1蛋白的表达进行检测,并进行临床病理相关性分析。结果ERK1、ERK2和CyclinD1在MM组织中表达较普通痣细胞痣组织明显升高(P<0.01),ERK1、ERK2和CyclinD1与MM的Clark分级和Breslow厚度无明显相关性,ERK1和ERK2与CyclinD1在MM组织中表达显著相关。结论MM中ERK途径得到了过度激活,并可能促使CyclinD1表达增加,因此它们在MM的发病机制中可能起到一定作用,而它们在普通痣细胞中仅在A型痣细胞表达增加。

Objective To investigate the roles of extracellular signal-regulated kinase (ERK)1, ERK2 and Cyclin D1 proteins in malignant melanoma (MM) and ordinary nevus. Methods By SP immunohistochemical method, the expression of ERK1, ERK2 and Cyclin D1 proteins was detected in 30 cases of MM and 13 cases of ordinary nevi. Results The expression of ERK1, ERK2 and Cyclin D1 proteins was higher in MMs than that in ordinary nevi (P < 0.01). The expression of ERK1, ERK2 and Cyclin D1 was not correlated with Clark level and Breslow thickness. The expression of ERK1 and ERK2 was correlated with that of Cyclin D1. Conclusions ERK pathway is activated in MM and may result in Cyclin D1 over-expression, while in ordinary nevi, only expression of type A nevus cell is increased.