Ⅲ型胶原在腹主动脉瘤及正常主动脉中表达的研究

Studies on the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues

目的研究Ⅲ型胶原 (typeⅢcollagen)在腹主动脉瘤 (abdominalaorticaneurysm ,AAA)与正常主动脉中的表达差异。方法应用逆转录 聚合酶链反应 (RT PCR)方法研究 5例AAA标本和 3例正常主动脉标本的Ⅲ型胶原的表达情况 ,并应用免疫组织化学的方法研究Ⅲ型胶原在AAA和正常主动脉中的差异。结果AAA与正常动脉 (NA)组织相比 ,其Ⅲ型胶原的表达明显增强 ,AAA/NA为7 2 5 1(P <0 .0 1) ,而免疫组化则证实在AAA组织中 ,Ⅲ型胶原粗大、紊乱 ,在正常组织中则比较规整。结论Ⅲ型胶原在AAA中的表达比正常主动脉明显升高 。

Objective To investigate the expression of type Ⅲ collagen in abdominal aortic aneurysm tissues and normal aortic tissues. Methods RT PCR and immunohistochemistry were applied to detect the expression of type Ⅲ collagen in abdominal aortic aneurysm (AAA) tissues( n =5) and normal aortic (NA) tissues( n =3) . Results Expression of type Ⅲ collagen was increased in AAA group compared with normal group with AAA/NA= 7 251( P <0 01).The structure of type Ⅲ collagen was abnormal in AAA compared with normal aortic tissues. Conclusion Expression of type Ⅲ collagen was increased in AAA cases.