肿瘤坏死因子-α基因多态性与乙型肝炎病毒宫内感染易感性的关系

Relationship between genetic polymorphism of tumor necrosis factor-α and susceptibility to intrauterine HBV infection

目的 探讨肿瘤坏死因子-α(TNF-α)基因-238位点G/A单核苷酸多态性与乙型肝炎病毒(HBV)宫内感染易感性的关系。 方法 应用实时荧光定量聚合酶链反应技术检测HBV标志物阳性母亲所生45例HBV宫内感染儿童(Ⅰ组)、8 5例宫内未感染儿童(Ⅱ组)和126例对照组儿童TNF-α基因-23 8位点G/A单核苷酸多态性。 结果 HBV宫内感染组TNF-α基因-238位点A等位基因频率显著高于HBV宫内未感染组(x2=6.797,P=0.009)和对照组(x2=9.51 3,P=0.002),HBV宫内未感染组和对照组之间差异无显著性(x2=0.047,P=0.828)。 结论 TNF-α基因启动子区23 8位点A等位基因与H B V宫内感染易感性相关,可作为检测其遗传易感性的标志之一。

Objectives To study the possible relationship between tumor necrosis factor (TNF)- α -238G/A gene polymorphism and the susceptibility to intrauterine HBV infection. Methods Two hundred and fifty-six children, including 130 infants born to HBsAg positive mothers were divided into two groups: forty-five children with intrauterine HBV infection (group I) and 85 children without intrauterine HBV infection (group Ⅱ), with a control group of 126. TNF-α -238G/A gene polymorphism was examined in all 256 children, by means of real-time quantitative fluorescent PCR. Results A significant difference of TNF- α -238A allele frequency was found between group I and group Ⅱ ( x2= 6.797, P = 0.009), and between group I and the controls group ( x2= 9.513, P = 0.002), but there was no significant difference between group Ⅱ and the control groups (x2= 0.047, P = 0.828). Conclusion This study found that genetic polymorphism of tumor necrosis factor- a was associated with intrauterine HBV infection.