人、鼠正常组织及人食管鳞状细胞癌组织中RFP蛋白的表达检测

The Expressive Detection for RFP Protein in Human, Mouse Normal Tissues and Human Esophageal Squamous Cell Carcinoma

目的:研究RFP(Ret Finger Protein,RFP)在人、鼠正常组织及食管鳞状细胞癌组织中的分布。方法:本研究运用 实时定量PCR方法并以18SrRNA作为内对照分析了RFP在人子宫颈、子宫内膜、睾丸、胃、食管和脑组织以及在鼠肺、肾、子 宫、卵巢、胸腺、心、肌肉、睾丸、皮肤、食管、脾、肝和肠等正常组织和人食管鳞状细胞癌组织中的表达。结果:RFP蛋白只在人 和鼠的睾丸组织中高表达,而在其他正常组织中低表达,在20例人食管鳞状细胞癌组织标本中检测到13例RFP表达上调, 其中9例有显著意义(P<0.05)。结论:由于RFP蛋白在正常组织中只高表达于睾丸组织,而约65％人食管鳞状细胞癌组织 中该蛋白的表达上调。上述结果提示RFP蛋白可能是治疗人食管鳞状细胞癌的一个潜在的分子靶点。

Objective: To investigate the distribution of RFP( Ret Finger Protein) in Human, mouse normal tissues and human esophageal squamous cell carcinoma. Methods: Real-time Quantitative RT-PCR with 18SrRNA as the internal standard was used to detect the expression of RFP in human normal tissues such as cervical tissue, endometrial tissue, tes-tis tissue, gastric tissue, esophageal tissue, brain tissue, human esophageal squamous cell carcinoma and mouse normal tissues such as lung tissue, kidney tissue, uterus tissue, ovary tissue, thymus tissue, heart tissue, muscle tissue, testis tissue, skin tissue, esophageal tissue, spleen tissue, liver tissue, colon tissue, intestinum tissue. Results: The highest level of RFP mRNA was only present in testis tissue among human and mouse normal tissues. In addition, the up-regula-tion of RFP protein was observed in 13 out of 20 human esophageal squamous cell carcinoma tissues, but only the upregu-lation of RFP in 9 out of 20 is significant(P < 0.05). Conclusions: Regarding to the expression of RFP limited to testis among human and mouse normal tissues and the up-regulation of RFP in 13 out of 20 human esophageal squamous cell carcinoma tissues, the RFP can be used as a potential molecular target for the development of new therapeutics for human e-sophageal squamous cell carcinoma.