摘要
AIIVI: To investigate the reversal effect of neferine on multidrug resistance in human gastric carcinoma cell line. METHODS: Cells of a human gastric cancer cells line, SGC7901, and its vincristine (VCR) -resistant variant, SGC7901/VCR, were cultivated with or without neferine and/or VCR. The cytotoxic effect of VCR was evaluated by the MTT assay. Cell apoptosis induced by VCR was determined by flow cytometry(FCM). The expression of P-glycoprotein (P-gp) and a multidrug-resistance-associated protein (MRP) in cells was examined by immunofluorescence and FCM. RESULTS: Neferine at the concentration from 2.5 μmol/L to 10 μmol/L had no cytotoxicity to SGC7901 cells, and its variant SGC7901/VCR cells. The ICso of VCR against SGC7901 and SGC7901/VCR cells was 0.059 μg/mL and 2.32 μg/mL, respectively, indicating that SGC7901/VCR cells were 39 times more resistant to VCR than its parent SGC7 901 cells. After treatment with neferine at concentrations of 2.5, 5 and 10 μmol/L, the IC50 of VCR to SGC7901/VCR cell line decreased to 0.340, 0.128 and 0.053 μg/mL, respectively,thus, increased the chemosensitivity by 6.8-, 18.1- and 43.8-fold, respectively. SGC7901/VCR cells were apoptosis resistant to VCR. Neferine (2.5, 5 and 10 μmol/L) promoted the VCR-induced apoptosis of SGC7901/VCR cells in a dosedependent manner. The expressions of P-gp and MRP were strongly positive in SGC7901/VCR cells, which were significantly down-regulated after treatment with neferine (10 μmol/L)for 24 h. CONCLUSION: Neferine reverses multidrug resistance of human gastric carcinoma SGC7901/VCR cells, which may be associated with the down-regulations of P-gp and MRP expression in SGC701/VCR cells.
AIM:To investigate the reversal effect of neferine on multidrug resistance in human gastric carcinoma cell line. METHODS:Cells of a human gastric cancer cells line,SGC7901, and its vincristine(VCR)-resistant variant,SGC7901/VCR, were cultivated with or without neferine and/or VCR.The cytotoxic effect of VCR was evaluated by the MTT assay.Cell apoptosis induced by VCR was determined by flow cytometry (FCM).The expression of P-glycoprotein(P-gp)and a multidrug-resistance-associated protein(MRP)in cells was examined by immunofluorescence and FCM. RESULTS:Neferine at the concentration from 2.5 μmol/L to 10 μmol/L had no cytotoxidty to SGC7901 cells,and its variant SGC7901/VCR cells.The IC_(50) of VCR against SGC7901 and SGC7901/VCR cells was 0.059 μg/mL and 2.32 μg/mL, respectively,indicating that SGC7901/VCR cells were 39 times more resistant to VCR than its parent SGC7 901 cells.Alter treatment with neferine at concentrations of 2.5,5 and 10 μmol/L,the IC_(50) of VCR to SGC7901/VCR cell line decreased to 0.340,0.128 and 0.053 μg/mL,respectively, thus,increased the chemosensitivity by 6.8-,18.1-and 43.8-fold,respectively.SGC7901/VCR cells were apoptosis resistant to VCR.Neferine(2.5,5 and 10 μmol/L)promoted the VCR-induced apoptosis of SGC7901/VCR cells in a dose- dependent manner.The expressions of P-gp and MRP were strongly positive in SGC7901/VCR cells,which were significantly down-regulated alter treatment with neferine(10 μmol/L) for 24 h. CONCLUSION:Neferine reverses multidrug resistance of human gastric carcinoma SGC7901/VCR cells,which may be associated with the down-regulations of P-gp and MRP expression in SGC701/VCR cells.
