用HPLC法测定水中氟等七种阴离子方法的探讨

Determination of inorganic anions in water by HPLC

:[目的 ] 研究同时测定水中阴离子的方法。 [方法 ] 应用高效液相色谱仪在化学键合固定相ODP柱上用邻苯二甲酸作流动相添加剂 ,在波长 2 6 6nm间接测定。 [结果 ] 方法检测限 (以S =2N计 )分别为 0 .0 0 7μg/mL(F-) ,0 .0 15 μg/mL(Cl-) ,0 .0 2 1μg/mL(NO2 -) ,0 .0 32 μg/mL(Br-) ,0 .0 2 7μg/mL(NO3-) ,0 .0 44 μg/mL(HPO32 -) ,0 .0 5 6 μg/mL(SO4 2 -) ,精密度试验RSD %为 1.0 0 %～ 1.73 % ,回收率为 86 %～ 112 %。 [结论 ] 本方法快速 ,简便 ,应用于自来水、矿泉水和纯水测定 。

Objective] To research method to determine seven inorganic anions in water. [Method] O-phthalio acid was used as a mobile phase additive for the chromatographic of inorganic analyses anions on a asahjsak ODP-50 column(125×4mm)phase (1.0mL/min)and indirect UV detection at wave length 266nm.[Results] The detection limits were 0.007 μg/mL(F-),0.015μg/mL(Cl-),0.021μg/mL(NO 2-),0.032μg/mL(Br-),0.027μg/mL(NO 3),0.044μg/mL(HPO 3 2- ),0.056μg/mL(SO 4 2- ).RSD was between 1.0% and 1.73%, and the recovery was between 86% and 112%. [Conclusion] The proposed method has been applied to tap water, mineral water adn pure water with satisfactory results.