摘要
目的 探讨健康人增龄过程中血小板内游离钙浓度的改变及其病理生理意义.方法 以Fura-z为钙敏感指示剂,应用荧光分光光度计(日立F-4000型)测定17名50～59岁、8名≥60岁男性血小板胞浆游离钙浓度,并以14名20～39岁健康男性为对照.结果 随年龄增高血小板游离钙明显增高,20～39岁血小板游离钙为169.66±36.46nmol/L;50～59岁为237.40±31.0(P<0.01),≥60岁为367.36±220.0(P<0.05).结论 血小板胞浆游离钙浓度随增龄而升高,并参与了衰老和老年人血液高凝性疾病的病理生理变化.
Objective To probe into the alteration of intraeellular free calcium concentration in platelets in the process of ageing and its pathophysiology significance. Methods The intracellular free calcium concentration in platelets was measured with Flourescence technique and Fura - 2 indicator. Results The concentrations of free calcium in platelets were 169.66 ± 36.46nmol/L in 14 young men aged 20 - 39, 237.40±31 .0nmol/L in 17 males aged 50 - 59, and 367.36 ± 220. 0nmol/L in 8 males over 60 (P < 0.01 and 0.05 respectively). Conclusions Concentration of intracellular free calcuim is increased with ageing, it may be one of pathophysiologic change in the ageing and in the disease of blood hypercoagulation of the elderly.
出处
《老年医学与保健》
CAS
1999年第1期19-20,共2页
Geriatrics & Health Care
基金
国家自然科学基金委员会资助(项目编号:39200140)
