格列齐特中亚硝酸盐的电化学测定法

Determination of nitrite in gliclazide by HPLC-ECD method

目的:建立高效液相-电化学(ECD)检测法测定格列齐特中亚硝酸盐(NO2-)的残留量。方法:阳离子色谱柱,醋酸盐缓冲液(pH为4.3)为流动相。结果:NO2-线性范围0.04～0.80μg,r=0.999 5,回收率为99.2％,RSD=1.7％(n=9)。结论:方法灵敏,简便,结果准确。

Objective: To establish a HPLC-ECD method for determination of nitrite remaining in gliclazide. Methods:A colum of Hypersil-SAX was used. The mobile phase was 13.61g of sodium acetate dissolved in water(pH4. 3) with phosphoric acid and diluted to 1 000mL. Results:The linear range was 0. 04 - 0. 80μg( r = 0. 999 5 ). The average recovery was 99. 2% RSD = 1. 7% (n = 9). Conclusion:The method was simple and accurate.