三元络合物比色法测定复方苦参洗剂中小檗碱的含量

Determination of bererine in compound Sophora flavescens lotions by three vari- ables complex chromatometry

目的:建立三元络合物比色法测定复方苦参洗剂中小檗碱的含量,为制剂质量控制提供依据。方法:小檗碱在pH值为6.7的溶液中,可与溴酚蓝、奎宁形成三元络合物,经氯仿提取,在610nm波长处测定吸收度。结果:小檗碱在0.8～4mg/L范围有良好的线性关系(r=0.999 9),平均回收率为98.65％,RSD为1.12％。结论:本方法准确,灵敏度好,可用于复方苦参洗剂等中药制剂中小檗碱的含量测定。

Objective: To establish three variables complex chromometry for the determination of berberine in compound Sophora flavescens lotions. Methods:The berberine formed three variables complex with bromophenol blue and quinine in pH6.7,then it was withdrawn with chloroform. The detection wavelength was 610nm. Results: The linear range of berberine was the 0. 8 ~ 4mg/L( r = 0. 999 9) ,the average recovery was 98.65% ,RSD was 1. 12% . Conclusion:The method had a good sensitive, and reproducibility. It can be used for determination of berberine in compound sophora flavescens lotions.