产后脂肪肝奶牛外周血浆孕酮、前列腺素F_2α变化规律的研究

Study on the changes of peripheral P4 and PGF_2α in fatty liver cows after calving

采用组织学方法检查了12头产后黑白花奶牛,以确定其脂肪肝的发生率。应用放射免疫法(RIA)测定受试牛分娩后外周血浆中孕酮和前列腺素F_2α的含量及其变化规律,从而探讨奶牛发生脂肪肝对繁殖力的影响。实验结果表明,产后7～12天内脂肪肝发病率为66.67%;脂肪肝患牛在产后第一次出出孕酮值明显升高的平均时间为22.6±3.7天,对照组正常奶牛(15±3天)显著延迟(P<0.05);产后1～29天两组牛的前列腺素F_2α水平没有明显差异。

The effects of fatty livre on fertility were studies in a total of 12 Friesian Cows. Fatty liver was assessed by liver biopsy. Meantime the concentrations of peripheral P_4 and PGF_2α were determined by radio immunoassay(R1A). The experiment showed. 8frien cows(66.67%) were diagnosed as 'having fatty liver 7～21 days after calving. The first rise of peripheral P_4 concentration after calving was 22.6±3.7 days for cows with fatty liver which was significantly longer than that of 15±3 days for the normal cows (P<0.05). Peripheral PGF_2α concentration during the periods of 1～29 days after calving wes not different between the two groups, PGF_2α concentration of normal cows and cows with fatty liver were in limit of 427±35 to 428±178 pg/ml and 419±81 to 508±67pg/ml, respectively.