非离子表面活性剂微乳的制备及抗菌性考察

Study on the preparation and self-preserving property of nonionic surfactant microemulsions

目的 :制备一种药用非离子表面活性剂空白微乳 ,并评价其抗菌性。方法 :采用拟三元相图考察表面活性剂、助表面活性剂及其质量比、添加剂、温度等对微乳区的影响 ;高速离心法和 37℃恒温 1个月考察微乳稳定性 ;抗菌试验考察其抗菌能力。结果 :油酸乙酯为油相、聚氧乙烯蓖麻油为表面活性剂、异丙醇为助表面活性剂的微乳区范围较大 ,可无限稀释 ;稳定性试验未见相分离 ;水相中的添加剂及温度的变化对微乳区范围无显著性影响 ;微乳均能有效杀灭绿脓杆菌、金黄色葡萄球菌、大肠杆菌、白色念珠菌 ,其中作用 8h后绿脓杆菌杀灭完全。结论 :本品能自身抗菌 ,有可能成为适合眼用、注射等的潜在给药载体。

OBJECTIVE To prepare a kind of medicinal non-ionic surfactant microemulsion and evaluate its self-preserving property. METHODS The effects of surfactants, co-surfactants and its mixing ratio,additives,temperature on micro-emulsion region were examined by using the pseudo-ternary phase diagram.The physical stability of the micro-emulsion was assessed by centrifugation at 4 000 g and by storage at 37 ℃ for one month. The antimicrobial activity of the micro-emulsion was tested using USP method for the examination of antimicrobial effectiveness in pharmaceutical preparations. RESULTS The extent of the region of the micro-emulsion was larger and the system can be diluted infinitly when ethyl oleate was used as the oil phase, Cremophor EL35 as surfactant, isopropanol as cosurfactant. The formation of the microemulsion was not dependent on the type of additives in water phase and the variation of temperature.No phase separation was observed in the stability testing.The micro-emulsion can kill effectively Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans and Escherichia coli.The viability of Pseudomonas aeruginosa decreased rapidly over a period of 8 h until no viable cells were observed.CONCLUSION The micro-emulsion is self-preserved, and is a kind of potent carrier for ocular and injection drug delivery system.