美蓝预防术后腹膜粘连的实验研究

Study of Methylene Blue in The Prevention of Postoperative Intraperitoneal Adhesion

目的 为研究美蓝的抗粘连作用效果 ,本文根据近年的文献报道 ,从中筛选出三种抗粘连作用相对明显的药物与其相对比 ,旨在为美蓝的进一步临床应用提供依据。实验方法 选 12 0只健康雄性Wistar大鼠 ,体重 2 80g-3 2 0g ,随机分为 6组 ,每组 2 0只。制成粘连模型后 ,按组于腹腔内分别注入相应浓度的尿激酶、美蓝、抑肽酶、甲基强的松龙、生理盐水各 2ml;术后 14天处死动物 ,盲法观察粘连程度并取标本送病理检查。结果 美蓝组、尿激酶组、抑肽酶组及甲基强的松龙组粘连程度均低于生理盐水组 ,其中 ,美蓝组及尿激酶组与生理盐水组比较 (P <0 .0 5 ) ,显示均有明显抗粘连作用 ;尿激酶组与美蓝组比较无明显差异。结论 尿激酶可减轻大鼠术后腹膜粘连程度 ,显示有抗粘连作用 ;美蓝有明显的抗粘连作用 ,其效果优于抑肽酶及甲基强的松龙 ,与尿激酶比较无明显差异 ;浓度为 0 .0 75 %的美蓝组与浓度为 0 .1%的美蓝组相比较 ,其抗粘连作用效果无明显差异。

Objective To study the effects of Methylene Blue on preventing adhesion and to provide evidences for its further use,comparing it with other three drugs which have apparent effects according to recent reports.Methods use 120 healthy wistar male-rats weighing between 280g and 320g each.The rats were randomly clallified into 6 groups by 20 in each.After producing adhesion model,inject into 2ml certain concentration of Droloinasum,Methy Prednisolone.Methylene Blue,Aprotinin and Natrii Chloridi respectively.After rats lysis on the 14th day. Observe the adhesion grades and send adhesional samples for pathological observasion.Results Obviously statistical difference was found among groups of Urokinasum,Methylene Blue,Aprotimin and Methy prednisolone:effects of Methylene Blue was much better than other groups.(P<0.05).Conclusions Methylene Blue was excellent in preventing intraperitoneal adhesion and its effect was better than Droloinasum, Aprotinin and Methyprednisolone.