HBV-DNA、乙肝病毒前S1抗原和乙肝病毒血清标志物的检测结果分析

Study of relativity between HBV-DNA 、Pre S1 and hepatitis B markers

目的 了解HBV -DNA、乙肝病毒前S1抗原和乙肝病毒血清标志物检测的关系及其临床应用价值。方法 对 183例HBsAg阳性标本和 3 0例HBsAg阴性对照采用荧光定量PCR进行HBV -DNA检测 ,采用ELISA进行乙肝病毒前S1抗原和乙肝病毒血清标志物检测 ,结果进行分析。结果 HBV -DNA在HBsAg( +)、HBeAg( +)组的检出率为 89.41% ,明显高于其它模式 (p <0 .0 1) ;乙肝病毒前S1抗原与HBV -DNA有明显正相关γ =0 .8111;乙肝病毒前S1抗原在HBeAg阳性组的检出率为 72 .94% ,明显高于其它HBVM模式 (P <0 .0 1)。结论 HBV -DNA的检测是目前临床检测乙肝病毒最灵敏、特异的方法 ,乙肝病毒前S1抗原能代替或补充HBV血清学系统和HBV -DNA的检测。

Objective To observe the relationship and value of HBV-DNA 、Pre S1 and hepatitis B virus markers.Methods HBV-DNA 、Pre S1 and hepatitis B virus markers were detected by using serum samples of 183 cases with HBsAg(+)as patient group and 30 cases with HBsAg(-) as comparison.The data were analysed .Results HBV-DNA positive rate in the HBsAg(+) and HBeAg(+) group was higher than that in the other hepatitis B virus markers groups (p<0.01), It was found that Pre S1 were correlated with HBV-DNA with the coefficient being 0.8111, Pre S1 positive rate in the HBeAg(+) group was higher than that in the other hepatitis B virus markers groups (p<0.01).Conclusions The detection of HBV-DNA is the most sensitive and specific way in hepatitis B virus clinical examination .hepatitis B virus Pre S1 can substitute or complement hepatitis B markers and HBV-DNA detection.