针刺对胃粘膜损伤家兔胃粘膜生长抑素及其受体基因表达的影响

Effect of Acupuncture on Gastro-mucosal Somatostatin and Its Receptor Gene Expression in the Rabbit with Gastric Ulcer

目的 :探讨针刺足阳明经穴对家兔胃粘膜损伤保护作用的机制。方法 :将动物随机分为正常组、模型组、针刺组 ,每组 8只。采用无水乙醇损伤造模法。针刺穴位取左侧“内庭”“解溪”“足三里”“梁丘”“天枢”“梁门” ,每日 1次 ,每次 3 0min ,连续 7d。采用RIA法检测胃粘膜生长抑素(SS)含量 ,采用RT PCR反应方法检测胃粘膜中SSR1 mRNA表达的强度。结果 :针刺组胃粘膜中SS含量、SSR1 mRNA表达水平明显低于模型组 (P <0 .0 1)。结论 :针刺足阳明经穴可减少生长抑素的生成 ,抑制家兔胃粘膜生长抑素受体基因表达强度 ,促进粘膜上皮细胞的增殖 ,加速损伤粘膜的修复 ,从而表现出良好的细胞保护作用。

Objective: To explore the underlying mechanism of acupuncture of acupoints of the Foot-Yangming Meridian in preventing the gastro-mucosa from injury in the rabbit with gastric ulcer. Methods: 24 New Zealand rabbits were evenly randomized into control, model and acupuncture groups. Gastric ulcer model was established by intragastric perfusion of absolute ethyl alcohol (2.5 mL/kg). “Neiting”(ST 44), “Jiexi”(ST 41), “Zusanli”(ST 36), “Liangqiu”(ST 34), “Tianshu”(ST 25) and “Liangmen”(ST 21) were punctured once daily, 30 min/time, and continuously for 7 days. Somatostatin (SS) contents in the gastric mucosal tissue were assayed with radioimmunoassay technique. SS receptor(R) 1 mRNA expression was detected with reverse transcription (RT)-polymerase chain reaction (PCR) technique. Results: Gastro-mucosal SS contents of control, model and acupuncture groups were 1852.4±361.7 mIU/mL, 2978.6±587.6 mIU/mL and 1800.2± 488.1 mIU/mL respectively; and the optical density integral values of SSR 1/cyclophilin in the 3 groups were 1.04±0.36, 2.56±0.25 and 1.07±0.08 separately, showing that in gastric ulcer rabbits, gastro-mucosal SS level and SSR 1 mRNA expression increased significantly in comparison with those of normal control group (P<0.01) and after acupuncture, SS level was lowered and SSR 1 mRNA expression was downregulated significantly (P<0.01). Conclusion: Acupuncture of acupoints of the Foot-Yangming Meridian can significantly reduce the production of SS and suppress the expression of SSR 1 mRNA in experimental gastric ulcer rabbits.