针刺对部分切断背根猫备用背根节c-fos表达的影响

Effect of Acupuncture on c-fos Expression in Dorsal Root Ganglia in Partially Dorsal Rhizotomized Cats

目的 :探讨针刺对部分切断背根猫备用背根节c fos表达的影响 ,为了解c fos与针刺促进脊髓可塑性的关系奠定基础。方法 :取成年健康雄性猫 10只 ,随机分 2组 :手术 7d组及针刺备用根 7d组 ,每组 5只动物。取各组术侧L6 背根节于 -2 0℃恒冷切片机上连续切片 ,片厚 2 0 μm ,分别用兔抗c fos( 1∶10 0 0 )抗体行免疫组化ABC法染色。观察并测量各组背根节中c fos蛋白的分布和单位面积c fos阳性神经元数。进行两样本均数比较的t检验。结果 :针刺备用根后 ,备用背根节中的c fos阳性神经元数与手术组比显著增加 (P <0 .0 5)。结论 :针刺备用根后c fos在备用背根节表达增加 ,提示c fos可能在针刺促进脊髓可塑性中发挥作用。

Objective: To explore the effect of electroacupuncture (EA) on expression of c-fos in the spared dorsal root ganglia (DRG) of partially dorsal rhizotomized cats so as to analyze the correlation between c-fos and the effect of acupuncture in promoting plasticity of the spinal cord.Methods: Ten adult cats were randomly and evenly divided into control group and acupuncture group. All the cats underwent unilateral section of L 1～L 5 and L 7～S 2 DRG with the L 6 spared. In acupuncture group, EA (98 pulses/min, duration of 30 min) was applied to “Zusanli”(ST 36) and “Xuanzhong”(GB 39), “Futu”(ST 32) and “Sanyinjiao”(SP 6) alternatively which are innervated by L 6. After surviving for 7 days, these animals were killed. L 6 DRG of every group was taken out to be cut into 20 μm sections that were then stained with immunohistochemical technique (ABC method). The distribution and the number of c-fos positive neurons in the spared DRG (L 6) of each animal were observed and counted under light microscope. Results: After acupuncture, the number of c-fos positive neurons (medium and small size neurons) in the spared DRG increased apparently than that of control group (161±31 vs 98±27, P<0.05), suggesting that the therapeutic effect of EA may be mediated mainly by the medium size and small size neurons in DRG and c-fos possibly plays a role in the process of acupuncture in strengthening the plasticity of the spinal cord. Conclusion: EA of acupoints may generate up-regulation of expression of c-fos in the spared DRG.