运动对TNF-α含量及其mRNA的影响被引量：12

Effects of Exercise on Concentrations of TNF-α, TNF-α mRNA in Rats

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摘要采用放射免疫技术 ,设计三种实验模型 ,即力竭游泳运动模型、递增负荷游泳训练模型、注射BSO(L-ButhionineSulfoximine丁胱亚磺酰亚胺 )排空GSH模型 ,观察在这三种实验模型下雄性SD大鼠血清TNF -α(细胞肿瘤坏死因子 -α)含量的变化 ,发现力竭运动后血清TNF -α含量增加 ,运动训练组TNF -α含量低于对照组。注射BSO组 ,其TNF -α含量与对照组相比具增加的趋势 ,但无统计学意义。长时间力竭运动机体TNF -α含量增加 ,以及活性氧产生增加 ,可能影响c -junmRNA和c -fosmRNA的表达量 ,进而影响转录因子AP - Serum TNF-α concentrations and TNF-α mRNA in liver are tested by using reverse transcription-polymerase chain reaction (RT-PCR) and radio-immunology technical. The results show that serum TNF-α concentrations elevate after exhausted exercise; TNF-α concentrations decrease after 10-week progressive training; TNF-α concentrations have a tendency of increase after the GSH is depleted; TNF-αmRNA expression in liver increase after one exhausted exercise; these results indicate that one strenuous exercise produces an increase in reactive oxygen species generation and in the proinflammatory cytokine TNF-α. These changes can activate the transcription factors of AP-1 and NF-κB.

作者毛丽娟许豪文

机构地区上海交通大学体育科研所华东师范大学体育与健康学院

出处《北京体育大学学报》 CSSCI 北大核心 2004年第7期930-932,共3页 Journal of Beijing Sport University

关键词力竭运动 TNF-Α MRNA 递增负荷运动训练氧化还原状态 exhaustive exercise TNF-α mRNA progressive training BSO

分类号 G804.7 [文化科学—运动人体科学]

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