亚硝基胍诱变选育酸性蛋白酶产生菌

Studies on the Screening of Strains withHigh Activity Acid Protease for Wool

利用亚硝基胍对野生黑曲霉DH -0 10进行诱变 ,使用自制的培养基筛选出 10株对羊毛细化具有效果的菌株 ,又通过摇瓶复筛出一株对羊毛细化具有明显效果的菌株并命名为DH -C40。同时设计了一种以羊毛作为底物的酶活力测定方法 ,用此法筛选出的菌株的最高产酶活力可以达到 62 0 μg/ml。

A high yield acid protease producing strain Asp.niger DH C40 was screened from its parent strain Asp.niger DH-010 by treating with N methyl N' nitrosoguanidine (NTG).The acid protease is good at decompose wool.Invented a new way to determine the activity of the enzyme for wool.Define 1ml enzyme decompose 1 μg wool in one hour as 1 unit activity of enzyme.A new screenging culture medium was designed.Using the new culture medium,10 strains which can produce the enzyme for wool were screened.Using the flask bottle to screen among the 10 strains,we got a high yield strain -DH C40.Using the new determine way,DH 40 can produce a high yield acid protease for wool,the activity of protease is 620 ug/ml .