水稻有机营养研究中完全及局部无菌培养方法探讨

Studies on totally and partially enclosed sterile culture methods used in the research of rice organic nutrition

在无菌培养室内就水稻有机营养研究中水稻苗完全无菌及局部无菌培养方法作了比较研究。结果表明,在每隔3d更换一次培养液的前提下,过大(2000mL)或过小(250mL)的培养容积对完全无菌水稻幼苗的生长影响相对较大,但是采用500mL和1000mL三角瓶培养的完全无菌水稻幼苗长势无显著差异;在50～1000mL范围内培养容积对局部(根)无菌水稻幼苗的生长影响不显著,可以采用易于操作的小容积(50mL)培养。培育后期局部无菌苗的生长状况明显优于完全无菌苗,且其具有较低的最终累积污染率。因此,在植物有机营养研究中采用局部无菌培养方法是可行的。

Comparative studies between totally enclosed sterile culture method and partially enclosed sterile culture method used in the research of rice organic nutrition were conducted in sterile chamber. The results were as followed, with the precondition of replacing hydroponic solution every other three days, too big (2000mL) or too small (250mL) culture volume had relatively more effects on the growth of totally sterile rice seedlings, but there were no significant differences between the growth status of totally enclosed sterile rice seedlings cultured in the triangular flask of 500mL and 1000mL; Culture volume within the range of 50 to 1000mL had no significant effects on the growth of partially enclosed sterile rice seedlings, but small volume (50mL) was recommended for its easily operational advantage. The growth status of partially sterile enclosed rice seedlings excelled to that of totally enclosed sterile rice seedlings at culture evening, furthermore, partially enclosed sterile rice seedlings had lower ultimate cumulative pollution ratio. So partially enclosed sterile method can be used in the research of plant organic nutrition for its superiority and feasibility.