HPLC测定利福定胶囊的含量

Determination of rifandin contents in Rifandin capsule by HPLC

目的 建立利福定胶囊的含量测定方法。方法 采用KromasilC18柱 (4 .6mm× 2 0 0mm ,5 μm) ,以甲醇 - 0 0 5mol·L-1乙酸铵溶液为流动相 ,进样 2 0 μl,检测波长 2 5 4nm。 结果 利福定在 0 0 5～ 0 5 4mg·ml-1范围内 ,呈良好的线性关系 (r =0 9999) ,平均回收率为 10 0 6 6 % ,RSD =0 82 %。结论 方法准确可靠 。

OBJECTIVE To establish an HPLC method to determine rifandin contents in the capsules. METHOD Analytical column was Kromasil C 18(4.6 mm×200 mm,5 μm). The mobile phase consisted of methanol -0.05 mol·L -1NH 4Ac(65∶35) and rifandin was detected at 254 nm. RESULTS The method proved to be linear in the range of 0.05-0.54 mg·ml -1(r=0.9999).The average recovery was 100.66% with RSD of 0.88%.CONCLUSION The method is accurate and reliable. It can be used for the quality control of Rifandin capsules.