可移植性小鼠网织细胞肉瘤(LⅡ)的光镜、电镜及细胞化学观察

Transplantble Mouse Reticulum Cell Sarcoma(L Ⅱ)——Study by Histochemistry, Light and Electron Microscopy

将小鼠网织细胞肉瘤LⅡ移植到近交系615小鼠的皮下和腹腔内,本实验对其病理学特征作进一步的观察。皮下移植的实体瘤呈结节状,组织学检查瘤细胞弥散分布,肿瘤异型性明显,瘤组织内间质和血管都很稀少,瘤细胞体积较大,多有胞浆突起,HE染色胞质淡红染或呈空泡状,偶见吞噬红细胞现象;细胞核的大小和形状不规则,核分裂象多见,有较多病理性核分裂象。腹水瘤细胞呈圆形或卵圆形。胞浆丰富,细胞核同实体瘤,电镜观察瘤细胞表面可见大量微绒毛,呈褶皱状及泡状突起;细胞质内有较多的溶酶体,线粒体和粗面内质网。肿瘤组织内嗜银纤维较多,或见弯曲纤细的网状纤维穿插于瘤细胞之间。非特异性酯酶细胞化学染色胞浆内呈弥漫强阳性。根据肿瘤的组织结构,细胞形态和超微结构特征,网状纤维和非特异性酯酶的存在,以及原代肿瘤的组织发生来源,进一步确认该瘤株是组织细胞型恶性淋巴瘤(组织细胞肉瘤)。

The tumor cells of mouse reticulum cell sarcoma NO_2(L Ⅱ)were transplanted into inbred 615 — strain mice both subcutaneously and intropitoneally. Transplantable tumor at the suboutaneous inoculation appeared a nodulle. There was a few stroma and blood vessels in the tumor tissues. The mlignant cells were large, exhibiting a diffuse growth pattern. The argyrophilic fibers in the tumors were abundant. The fine reticular fibers distributed between tumor cells. Ascitic tumor cells showed round or oval. The nuclear shape was irregular. The nuclear mitosises and some pathological mitosises were active. The surface of the ascitic tumor cells had numerous microvilli,a few blebby processes. There were many rough surfaced endoplasmic reticula, mitochondria, lysosomes in the cytoplasm of the malignant cells. Naphtol aacetate essterase histochemical stain revealed prominent posibility within the entire cyoplasm of the tumor cells. According to the characteristics of the histological sstructure, cellular morphology and ultrastructure, the proprty of the reticular fibers and ANAE,and the histologic origin of the tumor,authors suggested L Ⅱ may be malignant lymphoma of histocytic type.