祛风止痒口服液对豚鼠变应性接触性皮炎的影响及其作用机制研究

Inhibition of the Oral Liquids Expelling Wind and Stopping Itch on Allergic Contact Dermatitis in Cavy

目的研究祛风止痒口服液对豚鼠变应性接触性皮炎(ACD)的抑制作用,并探讨其作用机制。方法采用2,4-二硝基氯苯(DNCB)建立豚鼠ACD模型,观察口服不同剂量祛风止痒口服液对豚鼠耳肿胀的抑制作用。以RIA法检测ACD豚鼠产生白细胞介素-1,2,6(IL-1,2,6)和粒细胞-巨噬细胞集落因子(GM-CSF)的水平及祛风止痒口服液对这4种细胞因子的作用。结果口服不同剂量祛风止痒口服液均能明显抑制ACD豚鼠耳肿胀(P<0.01),降低ACD豚鼠血清IL-2、IL-6及GM-CSF水平(P<0.01或P<0.05),但小剂量组ACD豚鼠血清GM-CSF水平无明显变化(P>0.05);ACD豚鼠血清IL-1水平无明显变化(P>0.05)。结论祛风止痒口服液对豚鼠ACD有抑制作用,其作用机制可能是通过抑制IL-2、IL-6及GM-CSF等细胞因子的释放有关,而与血清IL-1水平无关。

Objective To investigate the mechanism of the Oral Liquid Expelling Wind and Stopping Itch inhibiting allergic contact dermatitis (ACD) in cavy ACD. Methods The cavy of ACD was used and induced by means of applying dinitrochlorobenzene (DNCB) on the cavy's skin and challenging DTH response with DNCB. Using this cavy inhibition of ear swelling was observed after oral administration of the Oral Liquids Expelling Wind and Stopping Itch in different doses. The levels of interleukin-1(IL-1), interleukin-2(IL-2), interleukin-6(IL-6) and Granulocyte-macrophage colony stimulating factor(GM-CSF) in the sera of these cavies were detected by RIA. Results Cavy's ear swellings were markedly suppressed in each dose group of the Oral Liquids Expelling Wind and Stopping Itch(P<0.01), decreasing the levels of IL-2, IL-6 and GM-CSF(P<0.01 or P<0.05) in serum of ACD cavy, but the level of GM-CSF in serum of the small dose group of ACD cavy was not decreased(P>0.05); the level of IL-1 in serum of ACD cavy was also not changed(P>0.05). Conclusion The Oral Liquids Expelling Wind and Stopping Itch has the function of suppressing the cavy ACD induced by DNCB. Its action mechanism may be related to the inhibition of the production and/or section of the cytokines such as IL-2, IL-6, GM-CSF, etc. It has no relation with the level of IL-1 in serum.