大肠癌凝集素受体的特异性和定位分布的研究

Studies on the Specificity and Location of Lectin Receptors in Human Large Bowel carcinoma

本文应用7种凝集素对82例大肠癌及40例非癌大肠粘膜进行了亲合组织化学研究,结果表明:PNA在大肠癌的阳性率是86.6%,正常大肠粘膜全为阴性;UEA—1在左侧大肠癌的阳性率是58.7%,左侧大肠正常粘膜不表达UEA—1受体;ConA在大肠癌的阳性率是82.9%,正常大肠粘膜阳性率为56.7%;DBA、SBA在大肠癌的阳性率分别比正常大肠粘膜减少52.4%和26.8%。作者认为PNA受体是大肠癌的较高特异性肿瘤标记,UEA—1、ConA、DBA和SBA四种凝集素受体对大肠癌也有一定意义,可能为肿瘤相关标记。癌细胞PNA、UEA—1和RCA受体的定位分布与大肠癌的分化和组织学类型有关,大肠癌凝集素结合反应与癌转移之间无必然联系,但PNA和UEA—1对淋巴结微小转移癌灶的检出有一定帮助。

Seven lectins were used to study 82 cases of large bowel cercinoma and 40 cases of noncancerous large bowel mucosa by lectin hisdtocnemical method. The results showd that peanut agglutinin (PNA) had higher positive rates in large bowel carcinoma but normal bowel mucosa didn't express PNA recentor. Normal mucosa of left large bowel didn't bind Ulex europaeus agglutinin-I(UEA-I),wherease 58.7% of cancers boud it. Positive rate of Conconavilin agglutinin(Con A)increased 26.2% in cancers than in normal large bowel mucosa. Positive rates of Dolichos biflorus agglutinin(DEA) and Soybean agglutinin (SBA) in malignant careinomas respectively decreased 52.4% & 26.8% than in normal large bowel mucosa. The authors considered that PNA receptor could had specificity for human large bowel carcinoma and that UEA-I,Con A,DBA receptors could be associated tumor markers. Location of PNA,UEA-1I and Ricinus comunis agglutinin (RCA) receptors might be associated with the histologic classification and differentiation degree of the large bowel carcinoma. No relationship between change of lectin receptors and metastases of the cancers was observed,but both PNA and UEA-I affinitive histochemistry were benificial to the detection of micrometastase in lymphnodes.