胃、十二指肠溃疡患者胃窦G细胞的免疫组化观察

An Immunohistochemical Study on G Cell in Gastric Antrum of Patients with Gastric and Duodenal Ulcer

本文用免疫组化方法(PAP法)对胃及十二指肠溃疡患者胃窦粘膜内的胃泌素细胞进行研究,包括在计数单位长度粘膜内G细胞密度及观察G细胞内分泌颗粒的数量、着色程度。结果为十二指肠溃疡及胃溃疡患者G细胞密度与对照组无差异。少数十二指肠溃疡患者G细胞密度显著增高,分泌颗粒密集,提示分泌功能亢进。但也有十二指肠溃疡患者G细胞密度与对照组无差异。十二指肠溃疡组内各病例间胃窦G细胞的密度及分泌颗粒数量的差异,支持十二指肠溃疡为多源性疾病的学说,胃溃疡组胃窦G细胞密度与对照组接近,说明其病因主要是胃粘膜保护因素减弱。

With immunohistochemistry (PAP method), G cell producing gastrin in antral mucosa of the biopsy specimens from 21 patients (9 with gastric ulcer and 12 with duodenal ulcer) was studied and contrasted with specimens from the gastric carcinomas.The study included the calculation of G cell density in per unit length in antral mucosa, the observation of the number and the staining degree of G cell's secret granules. The results showed that there was no significant difference from duodenal ulcers and gastric ulcers in comparion with contrasted group.In the few specimens from duodenal ulcer, the density of G cells and the secret granules were much higher, pointing out the hypersecretion of G cells, but in a few specimens, the density was lower than that in the contrasted group.The variation of the density supported that duodenal ulcer was a disease of multiply pathogenesis.The density of G cells in gastric ulcers was relatively equal to that of the contrasted group.It might demonstrate that the weakened protective factors were the important cause of gastric ulcers.