丹参对缺氧/复氧心肌的保护作用涉及百日咳毒素敏感的G蛋白

Involvement of pertussis toxin sensitive Gprotein in the cardioprotection by Salvia miltiorrhiza against anoxia and reoxygenation in rat ventricular cardiomyocytes

目的 研究丹参注射液对缺氧 /复氧心肌细胞的保护作用及其机制。方法 采用酶解分离成年大鼠心室肌细胞化学缺氧模型 ,用视频跟踪计算机系统、细胞内双波长钙荧光系统和光学法分别观察心肌细胞收缩力学、细胞内钙和乳酸脱氢酶(LDH)释放等指标。结果 丹参 (3g/L)处理后降低心肌细胞最大收缩和舒张速率、收缩幅度以及电刺激诱导的钙瞬态幅度。缺氧导致细胞收缩力和钙瞬态幅度降低、舒张末细胞长度缩短、舒张末钙水平增高 ,复氧后各指标有所回复。缺氧 /复氧引起LDH释放增多。用 3g/L的丹参处理后 ,缺氧 /复氧引起的心肌细胞最大收缩和舒张速率、收缩幅度和电刺激诱导的钙瞬态幅度高于单纯缺氧组 ,舒张末钙水平低于单纯缺氧组 ,LDH释放减少。预先用百日咳毒素处理 ,丹参引起的心肌保护作用被减弱。结论 丹参可能通过百日咳毒素敏感的G蛋白发挥对缺氧

OBJECTIVE To study the cardiopr otecti veeffect and mechanism of Salviami ltior rhiza (SM) .METHOD Contrac tion and intrace llularcal cium weredeter minedwit hvideotr acking system and spectr of luoromet ricmethod .The chemicalanoxic me thod wase mployed .Lact atede hydrog enase (LDH)release from cardio myocytes wasmeasur edtoevalu at etheca rdiaci njury .RE SULTS The±dL/dtmax ,dL of cellcon traction and theampl itude of [Ca2 + ]iin thec ardiom yocytes followingS Mtrea tment were decrea sed .Duri ngan oxia the±dL/dtmax ,dLandam plitudeof [Ca2 + ]iwered ecreased ,whil ethedia stoli cCa2 + leve lwasele vated whenc ompare dwithco ntrolgroup .Alltheco ntractilepar ametersan dthediast olicCa2 + leve lwereback towardpr etreatm entvalues duri ngreox ygenati on .Anoxia andreoxygen at ionincr easedL DHrelease.Aft erthe treatme ntwithSM (3g/L) ,±dL/dtmaxanddL ofcellcon tractionandt heam plitudeof[Ca2 + ]iwerehi ghera ndt hediast olic Ca2 + levelas wellasLDHr ele asew erelow erthant hose inanoxi a/reo xygenatio ngroup .Pretr eatmentw ithpert ussist oxinatten uatedthe cardia ceffec tofSM .CONCL USION The cardio protec tionbySM may bemediat edbypertussi stoxinse sitiveGp rotein .