不停跳体外循环心脏手术对血清S-100β蛋白表达的影响

Expression of serum S-100β protein in cardiac operation with cardiopulmonary bypass of beating heart

目的研究围手术期血清S-100β蛋白的表达,重点研究停跳与不停跳对血清S-100β蛋白表达的影响。方法23例体外循环下心脏手术患者,分为两组:停跳组和不停跳组。于体外循环转机前10min、转机后10min、转机末、手术后第24h等4个时段分别抽取颈内静脉血,离心后取血清测定每个标本的S-100β数值。结果(1)心脏手术期间,血清S-100β蛋白浓度随着体外循环的进行而不断变化。(2)停跳组体外循环时间的平均值(85.2±20.2min)较不停跳组(62.8±16.4min)长,但无显著性差异(P>0.05);相应地,停跳组转机末S-100β峰值的平均值(1.65±0.52μg/L)较不停跳组(1.59±0.84ug/L)高,但无显著性差异(P>0.05)。结论(1)体外循环可导致血清S-100β蛋白浓度的升高,且血清S-100β蛋白浓度与体外循环时间正相关。(2)停跳或不停跳体外循环心脏手术对血清S-100β蛋白的表达无影响。

Objective This study is to characterize serum levels of S-100β protein in patients undergoing cardiac operations with cardiopulmonary bypass , with the focus on expression of serum S-100β protein in cardiac operation with cardiac arrest and beating heart. Methods Twenty-three patients undergoing cardiac operation with cardiopulmonary bypass were divided into two groups: cardiac arrest group and beating heart group. The level of S-100β protein in jugular vein were measured during the perioperative period. Results (1)During cardiac operation, serum S-100β protein concentrations changed with the course of cardiopulmonary bypass. (2)At the termination of CPB, cardiac arrest group had longer average cardiopulmonary bypass time(85.2±20.2min), but no statistic difference from that of beating heart group(62.8±16.4 min )(P>0.05). Accordingly, cardiac arrest group had higher average S-100β levels (1.65±0.52μg/L), but were not statistically different from beating heart group (1.59±0.84μg/L) (P>0.05). Conclusion (1) Cardiopulmonary bypass can increase serum S-100β level , and they have positive significant correlation each other. (2) Whichever measure to be taken, cardiac arrest or beating heart, doesn’t have different effect on expression of serum S-100β protein.