激光拉曼光谱技术在生物分子DNA研究中的应用和进展

The Application and Development of Raman Spectroscopy on Biological Molecule DNA

激光技术的兴起使拉曼光谱成为激光分析中最活跃的研究领域之一,已被广泛地用于物质成分的分析和分子结构的鉴定。本文综述了拉曼技术在DNA研究中近年来的最新进展,包括:DNA的常规拉曼光谱分析;DNA的激光共振拉曼光谱分析;DNA在金属表面或电极上吸附行为的表面增强拉曼光谱研究;DNA的傅立叶变换拉曼光谱研究等。并对拉曼光谱技术在DNA等生物大分子领域中的研究前景做了进一步的展望。

The appearance and development of laser technology have made Raman spectroscopy one of the most attractive research fields in analysis using laser. Raman spectroscopy has been widely used in the analysis of material components and detection of molecular structure. In this review,some recent advances of Raman spectroscopy on DNA are summarized,including traditional Raman spectroscopy,Fourier transform Raman spectroscopy,laser resonance Raman spectroscopy,surface-enhanced Raman spectroscopy and so on. In addition,we also predicted the further application and development of Raman spectroscopy on DNA and other large biological molecules.