单味中药提取物对体外培养的黑素细胞酪氨酸酶基因和c-kit基因表达的激活作用

The Effect of Extracts from Chinese Medicines on Tyr and c-kit Gene Expression in Normal Melanocyte in Vitro

目的研究补骨脂、白芷、夏枯草、川芎和墨旱莲单味中药提取物对黑素细胞的酪氨酸酶基因和原癌基因c-kit蛋白表达的影响,以及对总蛋白合成的影响。方法采用蛋白印迹法检测了酪氨酸酶蛋白和干细胞因子受体kit蛋白含量的变化;595nm比色标准曲线测定黑素细胞可溶性总蛋白含量。结果白芷、补骨脂、夏枯草、川芎和墨旱莲对黑素细胞可溶性总蛋白合成和c-kit基因蛋白表达有促进作用,其中白芷和补骨脂对c-kit基因蛋白表达的促进作用为69.44%和58.43%;夏枯草、墨旱莲和川芎对酪氨酸酶基因的蛋白表达有一定的促进作用。结论在白癜风治疗中传统中药具有较好的疗效,可能与酪氨酸激酶受体蛋白含量的提高相关,即通过SCF/kit蛋白途径起作用,因此可以通过该途径进一步筛选更多治疗白癜风的单味中药。

Objective To study the effects of several Chinese traditional medicines (CTM) on cell proliferation, cell toxicity, gene expression of Tyr and c-kit in normal melanocytes cultured from Caucasians. Methods The TRP and KIT protein expression was detected by Western blotting. The total soluble protein of melanocytes was measured by spectrophotometer by its absorption at 595nm against the standard concertration curre. Results Angelica dahurica, Fructus psoraleae, Spica Prunellae, Ligusticum chuanxiong Hort, and Eclipta Prostrasta stimulated the expression of KIT and the synthesis of total protein. The stimulating effect of Angelica dahurica and and Fructus psoraleae on the KIT expression was 69.44% and 58.43% respectively. However, these medicines only had a limited effect on the expression of Tyr in melanocytes. Conclusions The therapeutic effect of CTM on vitiligo may be related with the SCF/KIT pathway. This study might provide an useful way to screen more Chinese medicine for the treatment of vitiligo.