复方花刺参黏多糖对球囊血管成形术兔血浆内皮素及血清一氧化氮的影响

Effects of compound Stichopus variegatus mucopolysaccharide on the levels of blood nitric oxide and endothelin after angioplasty in rabbits

目的探讨复方花刺参黏多糖对家兔髂动脉腔内成形术 (TA)后血浆内皮素及血清一氧化氮的影响。方法 5 0只新西兰家兔随机分为 4组 :花刺参组、辛伐他汀组、模型组和正常组。花刺参组、辛伐他汀组和模型组用球囊导管剥脱损伤髂动脉内皮后 ,喂高脂饲料 6wk ,髂动脉造影显示形成粥样硬化狭窄 .行TA后 ,即日均停喂高脂饲料改普通饲料 ,花刺参组和辛伐他汀组经胃管给药 ,模型组给等量生理盐水 ,分笼喂养 ,自由饮水。正常组仅给予普通饮食及假手术。各组动物于TA后 4wk空腹颈动脉同步采血行血浆内皮素、血清一氧化氮浓度测定。结果TA后 4wk ,模型组与正常组相比血浆ET浓度明显升高 ,而血清NO浓度明显降低 ,花刺参组和辛伐他汀组血浆ET浓度显著低于模型组 ,而血清NO浓度显著高于模型组。结论家兔髂动脉TA后ET增多 ,NO减少 ,存在血管内皮功能失调 ,而复方花刺参黏多糖通过调整ET和NO的平衡改善血管内皮功能。

Objective To investigate the effects of Stichopus variegatus mucopolysaccharide on plasma nitric oxide (NO) and serum endothelin(ET) after angioplasty in rabbits. Methods 50 New Zealand rabbits were randomly divided into four groups: Stichopus group, simvastatin group, model group and normal group. Endothelium of iliac arteries in Stichopus group, simvastatin group and model group were denuded by balloon catheter and the rabbits were fed with 2%cholesterol, 3%lard and 3%yolk mixed forage for six weeks, and then, atherosclerotic stenosis was showed by iliac angiography.When transluminal balloon angioplasty was done, from then on the feeds was changed from high cholesterol to ordinary forage. Stichopus (0.5g·kg -1·d -1) and simvastatin (0.5mg·kg -1·d -1) were given by gastric canal. While NaCl solution (0.9%) was given in model group. All rabbits were fed in separate cages and free drinking. After four weeks since transluminal balloon angioplasty, blood was collected from carotid artery to measure the changes of concentrations of endothelin(ET) and nitric oxide(NO). Examination of pathology by optical and electron microscope were done at same time. Results After four weeks since transluminal angioplasty, the concentration of plasm ET in model group increased significantly compared with the normal group(114.86±24.89 ng·L -1 vs 75.62±9.68 ng·L -1) and serum NO decreased significantly(37.50± 12.79μmol·L -1 vs 106.37±18.01 μmol·L -1).The concentration of plasm ET in model group was higher than that in the simvastatin groups and Stichopus groups(114.86±24.89 ng·L -1 , 81.08±14.71 ng·L -1 and 83.34±11.41 ng·L -1 ,respectively),and the concentration of serum NO decreased obviously(95.63±8.62 μmol·L -1,98.80±9.06 μmol·L -1 and 37.50±12.79 μmol·L -1,respectively). Conclusion After iliac arterial TA the concentrations of plasm ET were increased and the serum NO were decreased, the endothelial function fell into disorder. The Stichopus variegatus mucopolysaccharide had the effects of improving the endothelial function after angioplasty in rabbits by adjustment of plasm ET and serum NO.