摘要
以假单胞菌Y1为出发菌株 ,分别通过亚硝基胍 (NTG) ,Co6 0 ,UV(紫外 )诱变 ,采用透明圈法筛选 ,获得了产壳聚糖酶较好的突变菌株假单胞菌Y8,其所产酶活力达到 3.0U·ml- 1,酶活力提高近 6倍 ,并具有较好的遗传稳定性。 3种诱变方法中 ,UV诱变效果最好。
The conidia of Pseudomonas Migula Y 1were mutagenized by NTG, Co 60 and UV respectively. Chitosanase-producing mutant of Pseudomonas Migula Y 8 was obtained by screening with the method of transparent zones . Its chitosanase activity was 3.0 U·mL, -1 which has increased by six times approximately. The method of UV was better than others.
出处
《中国海洋药物》
CAS
CSCD
2004年第5期38-40,共3页
Chinese Journal of Marine Drugs