川芎嗪对大鼠肝细胞凋亡的影响

The Effect of Ligustrazine on Hepatocyte Apoptosis

目的 :采用四氯化碳 (CCl4)损伤性肝纤维化模型 ,观察川芎嗪对大鼠肝细胞凋亡的影响。方法 :雄性Wistar大鼠 40只随机分为 4组 :A组 (模型对照组 )、B组 (秋水仙碱组 )、C组 (川芎嗪组 ) ,采用 5 0 %CCl4和 5 %乙醇造模 ;D组为正常对照组 ,代之以等量生理盐水。 6周末处死动物 ,用TUNEL法检测各组肝细胞凋亡情况 ,生化分析仪测定血清肝功能指标 ,比色法检测肝组织匀浆MDA、GSH Px、Hyp ,免疫组化检测肝组织TGF β1的表达。结果 :川芎嗪能改善肝纤维化大鼠的肝功能 ,降低肝组织MDA、Hyp含量 ,增加GSH Px活性 ,使肝组织TGF β1的显色指数降低 ;凋亡细胞TUNEL标记显示 :模型对照组出现典型凋亡改变 ,川芎嗪治疗组未见明显凋亡改变 ,两组比较差异有显著性意义 (P <0 0 1)。结论 :川芎嗪可抑制纤维化大鼠肝细胞的凋亡 ,这可能是其抗肝纤维化的机制之一。

Objective:To study the effect of Ligustrazine on hepatocyte apoptosis.Methods:Adopt liver fibrosis induced by carbon tetrachloride in rats. Forty male Wistar rats were randomly divided into four groups:the normal contrast group, the model contrast group, the Ligustrazine group(80mg/kg),the Colchicine group(0.14mg/kg). Rats of different groups were given different drug by daily gastric feeding for six weeks. At the end of experiment, the following parameters were determinated:the liver function, the level of MDA、Hyp、GSH-Px, in situ TUNEL and immunohistochemical stain of TGF-β 1.Results:Ligustrazine could markedly decreased the levels of ALT、GGT、TBil、DBil,significantly prevented the increase of MDA、Hyp and decrease of GSH-Px,reduced the expression of TGF-β 1. The expression of hepatocyte apoptosis was significantly decreased.Conclusion:Ligustrazine could inhibited hepatocyte to apoptosis may be one of the anti-hepatic fibrosis mechanism.