结直肠癌患者8号染色体微卫星改变的研究

The study of microsatellite alterations on chromsome 8 in colorectal cancer

目的 研究结直肠癌 (colorectalcancer,CRC)患者 8号染色体微卫星改变的情况 ,探讨结直肠癌发病的分子机制。方法 应用聚合酶链反应 ( polymerasechainreaction ,PCR)、聚丙烯酰胺凝胶电泳及银染色方法 ,选取 8号染色体上 4个微卫星位点 ,对 34例结直肠癌患者的癌组织、癌旁组织及其相应正常组织的微卫星改变情况进行检测分析。结果 ① 34例患者中有 14例至少在一个位点发生微卫星改变 ,总检出率为 4 1 18% ,其中D8S135、D8S2 5 5、2 0 5 9%、17 6 5 %及 11 76 %。癌旁组织中未检测到微卫星改变。②发生两个以上位点改变的 10例 ,在有微卫星改变的患者中占 71 4 2 %。结论 在 8号染色体所选位点上存在较高频率的微卫星改变 ,提示可能存在与结直肠癌发病密切相关的基因 。

Objective: To study the frequency of MA on 8p, to investigate the function of MA during the progress of pathogenesis in colorectal cancer. Methods: By PCR, polyacrylamide gel-electrophoresis and silver staining, MA was detected at 4 microsatellite loci on chromsome 8p in tumor tissues, adjacent tissues and normal colorectum tissues excised from 34 cases with colorectal cancer. Results: 14 cases demonstrated MA at one or more loci, the total frequency was 41.18%. The frequency of MA on D8S135、D8S255、D8S511、D8S137was 29.41%、20.59%、17.65%、11.76%, respectively. None patient's adjacent tissues was found MA, ②MA positive at 2 and more loci was 10, the frequency among the total positive cases was71.42%. Conclusions: The most common MA occurrence at the 4 loci might imply the existence of the potential genes related to the carcinogenesis of colorectal cancer, and provide a effective index for early diagnosis of colonrectal cancer.