湖北土家族人群HLA-A*0201～0207与宫颈癌关联的研究

Association Analysis of HLA-A2 Alleles in the Population of Tu Nationality with Cervical Carcinoma in Hubei

目的 探讨湖北土家族人群HLA A 0 2 0 1～ 0 2 0 7与宫颈癌的关联性。方法 采用PCR/SSOP的HLA基因分型技术 ,利用一对引物扩增HLA A位点 2、 3外显子 ,并通过 31个地高辛标记的寡核苷酸探针分别与 192例中性外周血样本 ,其中包括 4 2例宫颈癌样本DNA扩增产物杂交 ,进行HLA A 0 2 0 1～0 2 0 77种等位基因分型 ;采用卡方检验 ,比较宫颈癌病例组和正常对照组中HLA A 0 2 0 1～ 0 2 0 7构成比的差异。结果 HLA A 0 2 0 2和HLA A 0 2 0 6在正常对照组和宫颈癌患者组的构成比有显著性差异 (P <0 0 5 ) ,相对危险度分别为HLA A 0 2 0 2OR =0 36 (95 %CI =0 13～ 0 95 ) ,HLA A 0 2 0 6OR =0 2 1(95 %CI =0 0 6～ 0 81)。结论 HLA A 0 2 0 2和HLA A 0 2 0 6对于宫颈癌发生的易感性可能有保护作用 ,本次研究未发现其余 5种等位基因与宫颈癌有关联。

Objective To explore the association between HLA -A*0201～0207 alleles and cervical carcinoma.Methods 192 women, including 42 women with cervical carcinoma were studied. 2, 3 extrons of HLA-A locus were amplified. By using PCR/SSOP gene typing, a single PCR amplification was performed in conjunction with 31 Dig-labeled oligonucleotide probes respectively. Allele-disease associations were assessed by χ 2 analysis.Results Associations were observed for HLA-A*0202 with an odds ratio of 0.36 (95% CI, 0.13～0.95) and for HLA-A*0206 with an odds ratio of 0.21 (95 %CI, 0.06～0.81), as compared with control group. Conclusion These findings suggest that HLA-A*0202 and HLA-A*0206 may be sufficient to confer protection against cervical carcinoma. However, the other 5 alleles bear no association with cervical carcinoma.