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荧光定量PCR技术在肿瘤研究中的应用 被引量：6

Application of Fluorogenic Quantitative PCR in Tumor Research
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摘要 荧光定量PCR技术具有简便、灵敏、准确等优点 ,目前已经在乙肝和性病的诊断和治疗中得到了广泛的应用 ,但是在肿瘤中的应用还处在研究和开发阶段 ,本文综述国内外近几年应用荧光定量PCR技术在肿瘤研究中的应用。 Fluorogenic quantitative PCR is simple, sensitive and accurate. It is widely used in the diagnosis of hepatitis B and venereal diseases, but less employed in tumor research. This article reviews the application of FQ-PCR in tumor research.
作者 陈文学 陈岳青 黄秀珍 邹学森
机构地区 江西省肿瘤医院
出处 《医学分子生物学杂志》 CAS CSCD 2004年第5期323-326,共4页 Journal of Medical Molecular Biology
关键词 荧光定量PCR技术 肿瘤研究 乙肝 诊断和治疗 性病 灵敏 综述 FQ-PCR tumor multidrug resistance gene
分类号 R730.54 [医药卫生—肿瘤] R759 [医药卫生—皮肤病学与性病学]
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参考文献19

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  • 2Summers KE, Davies AJ, Matthews J, et al. The relative role of peripheral blood and bone marrow for monitoring molecular evidence of disease in follicular lymphoma by quantitative real-time polymerase chain reaction. Br J Haematol, 2002, 118 (2): 563-6.
  • 3Lamarcq L, Deeds J, Ginzinger D, et al. Measurements of human papillomavirus transcripts by real time quantitative reverse transcription-polymerase chain reaction in samples collected for cervical cancer screening. J Mol Diagn,2002, 4 (2): 97-102.
  • 4Burger H, Foekens JA, Look MP, et al. RNA expression of breast cancer resistance protein, lung resistance-related protein, multidrug resistance-associated proteins 1 and 2,and multidrug resistance gene 1 in breast cancer: correlation with chemotherapeutic
  • 5Kolomietz E, Marrano P, Yee K, et al. Quantitative PCR identifies a minimal deleted region of 120 kb extending from the Philadelphia chromosome ABL translocation breakpoint in chronic myeloid leukemia with poor outcome.Leukemia, 2003, 17 (7): 1 313-23.
  • 6Ueno H, Yoshida K, Hirai T. Quantitative detection of carcinoembryonic antigen messenger RNA in the peritoneal cavity of gastric cancer patients by real-time quantitative reverse transcription polymerase chain reaction. Anticancer Res, 2003, 23 (2C): 1 70
  • 7Shimizu F, Nakayama J, Ishizone S, et al. Usefulness of the real-time reverse transcription-polymerase chain reaction assay targeted to alphal, 4-N-acetylglucosaminyltransferase for the detection of gastric cancer. Lab Invest,2003, 83 (2): 187-97.
  • 8Gelmini S, Tricarico C, Petrone L, et al. Real-time RTPCR for the measurement of prostate-specific antigen mRNA expression in benign hyperplasia and adenocarcinoma of prostate. Clin Chem Lab Med, 2003, (3): 261-5.
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医学分子生物学杂志
2004年 第5期

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