Hsp70和PCNA在NIH小鼠前胃癌变过程中的表达及意义

Expression and significance of Hsp70 and PCNA during the development of forestomach carcinoma in NIH mice

目的 :研究N 亚硝基 肌氨酸乙酯 (N nitrososarcosineethylester,NSEE)诱导NIH小鼠前胃癌变过程中 ,热休克蛋白 70 (heatshockprotein 70 ,Hsp70 )和增殖细胞核抗原 (proliferatingcellnu clearantigen ,PCNA)的表达及意义。方法 :将 14 4只小鼠分为6组 ,通过用NSEE灌胃建立小鼠前胃癌模型。每隔2wk处死 2 4只小鼠 ,取其前胃用免疫组化ABC法 ,对小鼠前胃癌变进程中细胞形态的改变 ,Hsp70和PCNA的表达进行跟踪研究。结果 :在NSEE诱癌过程中 ,Hsp70的表达呈现马鞍型 ,而Hsp70的表达一直呈增强的趋势。给药后 5 6、70d ,与对照组比较差异显著 (P <0 .0 5 ) ,84d差异极显著(P <0 .0 1)。PCNA的表达随着诱癌进程呈现递增趋势 ,给药后 4 2、5 6d ,与对照组比较差异显著 (P <0 .0 5 ) ,70、84d差异极显著 (P <0 .0 1)。Hsp70与PCNA的表达呈正相关关系 (r=0 .98,P <0 .0 1)。结论 :在诱发NIH小鼠前胃癌变过程中 ,Hsp70、PCNA的表达均呈现升高趋势 。

AIM: To explore the expressions and significance of heat shock protein 70 (Hsp70) and proliferating cell nuclear antigen (PCNA) in the development of forestomach carcinoma in NIH mice induced with N-Nitrososarcosineethylester(NSEE). METHODS: 144 mice were divided into 6 groups and their stomachs were infused with NSEE. Then 24 mice were killed every two weeks and their forestomachs were used for the study of the expressions of Hsp70 and PCNA by immunohistochemical staining. RESULTS: With the progress of carcinogenesis, the expression pattern of Hsp70 was up→down→up, but the expression trend was still increasing. On day 56 and day 70 after NSEE treatment, the expression level of Hsp70 was higher than that of control (P<0.05). On day 84, the expression level of Hsp70 was markedly higher than that of control(P<0.01). The expression of PCNA increased gradually with the carcinogenesis. As compared with the control, stronger expression was found on day 42 and day 56 (P<0.05) and notably stronger expression on day 84(P<0.01). The expression of Hsp70 was positively correlated to that of PCNA(r=0.98, P<0.01). CONCLUSION: In the develepment of forestomach carcinoma of NIH mice, the expressions of both Hsp70 and PCNA increased and were positively correlated with each other.