AD7C-NTP基因的染色体定位及其产物的跨膜区预测

The chromosomal location of AD7C-NTP gene and the prediction of transmembrane domains of its deduced protein

目的 :对AD7C NTP基因进行染色体定位 ,预测其编码产物的跨膜区。方法 :用Blat服务器进行AD7C NTPmRNA序列与基因组DNA序列的比对。借助PHDhtm、TMHMM2 .0、HMM TOP2 .0、SMART和PSORT等服务器 ,预测AD7C NTP基因编码产物的跨膜特征及其亚细胞定位。结果 :AD7C NTP基因定位于 1p36 .11负链 ,无内含子序列。AD7C NTP基因编码产物具有 3个跨膜区 ,可能定位于过氧化物酶体。结论 :对AD7C

AIM: To characterize the chromosomal location of AD7C-NTP gene and predict the transmembrane domains and sub-cellular location of its deduced protein. METHODS: The AD7C-NTP mRNA sequence was alignmented with human genomic DNA sequence by Blat server. The transmembrane domains and sub-cellular location of AD7C-NTP protein were predicted by using PHDhtm, TMHMM2.0, HMMTOP2.0, SMART and PSORT servers, etc. RESULTS: The AD7C-NTP gene located in minus strand of 1p36.11, without intron. The AD7C-NTP protein was predicted to have 3 potential transmembrane domains and locate on peroxisome’s membrane. CONCLUSION: Bioinformatics analysis of the AD7C-NTP gene and its deduced protein provides valuable clues for further gene cloning and study of function.